Never certain about anything regarding the AIO; I don't have one. I think you might be confusing balancing with calibration.
Balancing happens automatically every time the battery reaches near full. Think of the cells as buckets left out in the rain. One will always reach the full mark first, and the buckets each have a special little tap to let a tiny bit of water out. Using the taps you can make sure all the buckets get filled properly without any overflowing. Balancing can only be done at the "top", because when the buckets are partly filled they look the same, and when they are empty, you can't let water out!
Calibration is deliberately emptying all the buckets, and then filling them all to see how much they hold. The measurement is recorded and used to estimate the % full. The estimate will be more accurate if the system is filled and emptied frequently.
You can use my app to check the capacity recorded at the last calibration, and to see the cell balancing when the battery is full.